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We began our research on western
huckleberries and bilberries in earnest in 1994 at the
University of Idaho Sandpoint Research & Extension
Center. Our reasons are obvious. Sandpoint sits near the
center of diversity for mountain huckleberry and is home
to several huckleberry processors. The mountains around
the Center are covered with five species of western
huckleberries and the culture of the area heavily
emphasizes huckleberries.
From an economic perspective, north
Idaho and the surrounding region are transitioning from
an economy based on logging, traditional agronomic
crops, and mining to an ecotourism-based economy. The
myriad huckleberry products commercially available are
perfect complements to the tourism industry, providing a
uniquely Northwest product and experience.
Environmentally, western huckleberries
and bilberries are not threatened or endangered.
Supplies from wild stands are, however, dwindling due to
loss of forest habitat, forest and fire management
policies, and road closures to protect endangered
species, such as woodland caribou and grizzly bear. With
dwindling supplies and vastly increased demand, we were
seeing overharvesting in some areas and insufficient
crops to meet commercial demand, recreational picking,
and tribal uses.
Our research has two basic goals:
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Protect and enhance existing wild
stands for tribal uses, home consumption and
recreational picking, and small-scale processors.
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Produce huckleberries from managed
forest stands and in cultivation, as we do for
eastern lowbush and northern highbush blueberries to
meet demands for large scale processors and for
export.
We are presently working in four general
areas: cultivar development, propagation, environmental
studies, and development of production systems.
As of 2007, there were no named
varieties of western huckleberries, other than a few
ornamental evergreen huckleberries. This lack of
improved, vegetatively-propagated varieties is a
significant barrier to an industry based on producing
huckleberries and bilberries from managed stands or in
cultivation.
Modern fruit production is nearly all
based on named varieties, often called cultivars
(cultivated varieties). These varieties represent high
quality, known production and processing requirements,
and predictability. For example, an apple grower
planting Gala or Granny Smith has an abundance of
information available on how to manage these varieties.
Packers and processors know how to handle them and
consumers know what to expect in terms of appearance,
flavor, and suitable uses. This predictability comes
because the varieties characteristics are fixed. A new
Granny Smith tree is propagated by budding or grafting
from n established tree. Theoretically, every Granny
Smith in the world is genetically identical. In other
words, a clone.
Note that a clone simply means a plant
that has been vegetatively propagated using tissue from
a stock plant. The coleus or philodendrons your
grandmother rooted in a glass of water on the windowsill
were clones. The red delicious apple you bought at your
local nursery was a clone. The terms "clone" and
"genetically modified organism" have very different
meanings.
Uniformity, however, is lost when plants
are propagated sexually through seed production. Every
seedling in genetically unique. Some fruit crops, like
peaches or black currants, breed rather true to seed.
The seedlings pretty closely resemble the parents.
Western huckleberries, like their blueberry cousins,
show great variability in the seedlings' plant and fruit
characteristics. Breeding is a numbers game. The fact
is, most seedlings do not meet commercial standards for
plant habit, yields, or fruit quality. As a fruit
breeder, if I obtain one named variety out of one
thousand seedlings, I consider myself ahead of the game.
An average of one in ten thousand is often closer to the
average. That means producing many crosses, and
critically evaluating tens of thousands of seedlings to
find the very best.
Our cultivar development program starts
by obtaining seed for the species of interest. We have
collected seed from throughout the northwestern United
States and western Canada, and have seed from colleagues
collected across North America, Asia, and Europe. We
plant out the seed and evaluate the plants over about
seven years. We typically see fruit in three to five
years and the plants are usually large enough to select
seven years from sowing. The very best plants may become
early generation varieties, although most serve as
parents for controlled crosses. Their progeny (called
F1s) go through the same evaluation process. Needless to
say, breeding huckleberries and bilberries is a
long-term, very intensive process.
At the Sandpoint Research & Extension
Center, we presently are developing varieties of dwarf
huckleberry, Cascade huckleberry, bilberry, mountain
huckleberry, oval-leaved bilberry, and alpine bilberry.
We are also making some interspecific hybrids by
crossing one species with another (this occurs in nature
and we have collected many natural hybrids). Many modern
fruit varieties represent hybrids of several species.
All of our breeding at Sandpoint is done
conventionally using cross pollination. For security
reasons, we do not work with genetically modified
organisms at Sandpoint. While we are studying crosses
between western huckleberries and domestic blueberries,
plants from those crosses are intended for the blueberry
industry. Any huckleberry varieties released from
our program represent either single species or hybrids
of western huckleberry and bilberry species.
Although the genetics are very
complicated, the breeding process is simple. We choose
those plants that best meet our criteria of perfect
commercial plants There are no perfect plants, of
course). For example, I might have a plant that has a
good shape and produces large numbers of berries having
excellent flavor, but the berries are small. I would
choose to cross that plant with ant other desirable
plant that has particularly large fruit.
We start by collecting pollen from the
flowers using an electric engraving pen that vibrates at
about the same rats as bumble bee wings. We touch the
pen to a flower and catch the falling pollen in a small
glass bottle. Huckleberry flowers produce very little
pollen and we have to collect from many hundreds of
flowers to obtain enough pollen for breeding.
Flowers to be pollinated have their
petals and stamens (male organs) removed with a pair of
sharp forceps. We remove the petals and stamens (a
process called emasculation) just before the flower
opens. We then wait 24 to 48hours for the stigma (female
organ) to become receptive and coat the end of the
stigma with pollen from the donor plant. Abut two months
later, we harvest the ripe fruits and extract the seed.
Then the evaluation process starts all over again.
As of November 2007, we have nearly 100
early or advanced selections of western huckleberries
and bilberries. A handful of those selections are ready
for testing in other areas. We have a small network of
cooperating scientists, commercial nurseries, and fruit
growers that we have worked with for many years. When we
feel we have a selection that meets commercial
standards, we send out planting stock to our cooperators
for testing in their areas, usually for at least three
years.
Plants that continue to meet commercial
standards may then be patented by the University of
Idaho, named, and released to the public. In the Pacific
Northwest, fruit releases are usually done jointly with
the University of Idaho, Oregon State University, and
Washington State University. Nurseries that are
interested in the varieties may become licensed to
propagate those varieties, and the plants become
available to the public.
As you might guess, we receive many
requests for planting stock. Unfortunately, we cannot
provide plants outside of our network. First, we do not
have the resources necessary to propagate large numbers
of plants for distribution to the public. Our focus must
be on cultivar development and testing. Also, for
reasons involving plant patent laws, we must strictly
control distribution of our selections until they are
officially released.
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Knowing how to propagate our research
plants is, of course, very important. We have developed
and published seed propagation methodologies for
mountain huckleberry. We will soon publish seed
propagation methodologies for dwarf huckleberry, Cascade
huckleberry, oval-leaved bilberry, and red huckleberry.
In general, these species do not require stratification
or scarification of the seed before sowing.
Vegetative propagation methodologies are
required for the development of clonal varieties.
Unfortunately, mountain huckleberries have proven very
difficult to propagate by rooting stem cuttings. Rhizome
cuttings root well, but are slow and collecting the
rhizomes can damage or kill the stock plant. Some other
western huckleberries are also difficult to propagate
using traditional techniques.
Our primary vegetative propagation
methodology is tissue culture, also called
micropropagation or in vitro culture. Here we take a
single bud from a stock plant, sterilize the tissues,
and place it into a medium that provides nutrients,
moisture, and plant growth regulators (similar to animal
hormones) inside a sterile glass or plastic container.
Depending on how we treat the tissue, we can form many
new shoots, with or without roots. These microshoots are
then transplanted to potting soil in a high humidity
chamber where the shoots form roots. Several months
later, the plants are ready
to transplant to a larger container.
Details on
our micropropagation techniques have recently been
published. The citation is:
Barney, D.L., O.A. Lopez, and E. King. 2007.
Micropropagation of cascade huckleberry, mountain
huckleberry, and oval-leaf bilberry using woody plant
medium and Murashige and Skoog medium formulations.
HortTechnology 17 (3):279-284.
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Graduate student Omar Lopez selecting plants for
cloning. |

Explants are taken from the stock plants. |

Leaves are carefully removed so as not to damage
the buds. The explants are surface sterilized. |

The
explants are placed into sterile culture tubes
with nutrients and growth regulators. Note the
microshoot that has formed. |
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Microshoots are transferred to larger containers
where they are multiplied and allowed to
elongate. |

When
about two inches long, the microshoots are
transplanted to potting soil in a humidity
chamber. |

A
rooted microshoot ready for transplanting. |

Mature, clonally propagated western
huckleberries and bilberries. |
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In order to manage or cultivate these
plants, we must know what they require in terms of the
environment. Ongoing studies at the SREC involve how
huckleberries and bilberries respond to shading, various
soils, and bark rooting and growing media.
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Shade
cloth-covered frames used to evaluate plant
responses to levels of shading. Shade levels
range from full sun through 70% shade. |

Raised beds containing different soils. Awaiting
spring planting of huckleberries and bilberries
to determine adaptability to various soil types. |
We are presently testing huckleberry and
bilberry production systems using raised soil beds, bark
beds, field cultivation, and undercropping under a
simulated forest canopy. For more information on our
production methods, visit our
Growing Huckleberries page.
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