Gustin Lab
Agricultural Biotechnology Building

Empigen Lysis Buffer

Stock Solution Final Concentration For 10 mL 30% Empigen BB (Calbiochem) 2% 666 m L 1M EDTA 4 mM (1 mM from tablet) 40 m L 100 mM PMSF 0.1 mM 10 m L Complete Mini Protease Inhibitors (Roche)   1 tablet PBS 1X 9.28 mL

References: Lowthert et al. (1995) Biochem Biophys Res Comm. 206:370-9. Zhou et al. (1999) JBC. 274:12861-6.