| Name: |
| Carolyn Hovde Bohach |
| Title: |
| Professor and Director of Idaho NIH INBRE |
| Degree: |
| Ph.D. University of Minnesota |
| Phone: |
| (208) 885-5906 |
| Fax: |
| (208) 885-6518 |
| Email: |
| cbohach@uidaho.edu |
| Lab/Office Location: |
| Life Science South, Room 148 |
|
|
| Research Interests: |
| |
|
My laboratory works on the enterohemorrhagic Escherichia coli (EHEC), which include the infamous O157:H7 serotype. Ingestion
of these pathogenic bacteria can cause bloody diarrhea and the life-threatening hemolytic uremic syndrome. Healthy cattle are a
reservoir for these pathogens and contaminated foods of bovine origin are most often implicated in human disease.
|
| |
|
Projects include structure-function analysis of an EHEC protein exotoxin called Shiga toxin and investigations of the relationship
between healthy cattle and EHEC. Molecular and biochemical techniques are used to relate the structure of Shiga toxin to its
biological activities. For example, specific amino acids that comprise the catalytic center, that hold the A and B toxin subunits
together, or that signal translocation of the oxin through target cells have been identified and are being studied. Microbiological
and molecular genetic techniques are used to identify pre-harvest management practices that may reduce the risk of EHEC-positive
cattle from entering our food chain. Development of a vaccine to prevent cattle from carrying these human pathogens is also underway.
In addition, we are investigating the possibility that E. coli O157:H7 and other Shiga toxin-producing E. coli may
play beneficial roles in ruminant animals. To this end, we have shown that Shiga toxin has antiviral activity against bovine
leukemia virus.
|
| |
| Selected Publications: |
| |
John, M, RW Griffin, B Krastins, H Sheng, DA Sarracino, CJ Hovde, SB
Calderwood, IT Kudva. 2007. Microbial protein-Antigenome Determination
(MAD) Technology: A Proteomics-based Strategy for Rapid Identification of
Microbial Targets of Host Humoral Immune Responses. In Press.
Lim JY, H Sheng, K Seo, YH Park, and CJ Hovde. 2007. Characterization of an
E. coli O157:H7 plasmid O157 deletion mutant and its survival and
persistence in cattle. Appl. Environ. Microbiol. 73:In Press.
Li J and CJ Hovde. 2007. Bovine gene expression profiles at the rectoanal
junction mucosa during carriage of E. coli O157:H7. Appl. Environ.
Microbiol. 73: In Press.
Lim JY, J Li, H Sheng, TE Besser, K Potter, and CJ Hovde. 2007. Colonization
of Escherichia coli O157:H7 at the recto-anal junction of long-
duration culture-positive cattle. Appl. Environ. Microbiol.
73:1380-82.
Ferens, WA, M Halver, K Gustin, T Ott , and CJ Hovde. 2007. Differential
sensitivity of viruses to the antiviral activity of Shiga toxin 1 A subunit. Vir.
Res. 125:104-8.
Ferens, WA and CJ Hovde. 2007. The non-toxic A subunit of Shiga toxin
type 1 prevents replication of bovine immunodeficiency virus in infected cells.
Vir. Res. 125:29-41.
Sheng, H, JY Lim, HJ Knecht, JL Li, and CJ Hovde. 2006. The role of
Escherichia coli O157:H7 virulence factors in colonization at the
bovine terminal rectal mucosa. Infect. Immun. 74:4685-4693.
Sheng, H, HJ Knecht, IT Kudva and CJ Hovde. 2006. Application of
bacteriophage to control intestinal Escherichia coli O157:H7 in
animals. Appl. Environ. Microbiol. 72:5359-66.
Davis MA, H Sheng, J Newman, DD Hancock, CJ Hovde. 2006. Comparison of
a waterless hand hygienepreparation and soap and water hand washing to
reduce coliforms on hands in animal exhibit settings. Epidem. Infect 22:
1-5.
Ferens, WA, R Cobbold, CJ Hovde. 2006. Intestinal Shiga toxin-producing
Escherichia coli mitigate bovine leukemia virus infection in
experimentally infected sheep. Infect. Immun. 74: 2906-2916.
Dobbin, HS, CJ Hovde, CJ Williams, and SA Minnich. 2006. The
Escherichia coli O157 flagellar regulatory gene (flhC) and
not the flagellin gene (fliC) impacts colonization of cattle. Infect.
Immun. 74:2894-2905.
Davis, MA, DH Rice, H. Sheng, DD Hancock, TE Besser, R Cobbold, CJ Hovde.
2005. Comparison of rectoanal junction mucosal swab and fecal culture for
detection of Escherichia coli O157:H7 in dairy heifers. Appl. Environ.
Microbiol. Submitted.
Davis, MA, K.A. Cloud-Hansen, J. Carpenter, and C.J. Hovde. 2005.
Escherichia coli O157:H7 in the environments of culture-positive
cattle. Appl. Environ. Microbiol. 71: 6816-22.
Sheng, H, MA Davis HJ Knecht, DD Hancock, and CJ Hovde. 2005.
Characteristics of a Shiga toxin-, intimin- and EHEC hemolysin-producing
Escherichia coli ONT:H25 commonly isolated from healthy cattle. J
Clin. Microbiol. 43:3213-20.
Yoon, JW, JY Lim, YH Park, and CJ Hovde. 2005. Involvement of the
Escherichia coli O157:H7 pO157 ecf operon and lipid A
myristoyl transferase activity in bacterial survival in the bovine
gastrointestinal tract and persistence in farm water troughs. Infect Immun.
73:2367-78.
Ferens, WA, LJ Grauke, and CJ Hovde. 2004. Shiga toxin 1 targets bovine
leukemia virus-expressing cells. Infect. Immun. 72:1837-40.
Yoon, JW, SA Minnich, JS Ahn, YH Park, A Paszcynski, and CJ Hovde. 2004.
Thermoregulation of Escherichia coli O157:H7 pO157 ecf operon and
lipid A myristoyl transferase activity involves intrinsically curved DNA. Mol.
Micriobiol. 51:419-435.
|
| |
|