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Role of Hsp90 in polarized cell morphogenesis in S. cerevisiae and C. albicans |
Co-P.I. Jill Johnson
Abstract |
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The abundant cytoplasmic molecular chaperone Hsp90 and its co chaperones are critical for the activity of steroid receptors,
oncogenic tyrosine kinases and additional diverse proteins involved in signaling pathways and cell cycle control. Hsp90 and
co chaperones interact in an ATP dependent pathway that facilitates the folding of substrate proteins to their native state.
Hsp90 inhibitors have been shown to slow the ability of Candida albicans to develop drug resistance. A focus of our
work is to characterize the interaction of Hsp90 with yeast specific proteins, first in S. cerevisiae and then in
C. albicans. We developed an assay that allows us to isolate native yeast Hsp90 complexes that contain both known co
chaperone proteins and novel presumed client proteins. Using peptide mass mapping we identified four novel Hsp90 interacting
proteins and confirmed another interaction. In our efforts to determine how Hsp90 interacts with diverse client proteins, we
will examine the interaction of Hsp90 and co chaperones with these and other proteins in order to develop a more comprehensive
picture of the in vivo mechanisms of the Hsp90 molecular machine.
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